SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-29 01:31:57

_ec

description

Ser/Thr kinase, controls translation and DNA integrity during spore development

Ser/Thr kinase, controls [[category|Translation]] and DNA integrity during spore development

locus

BSU00660

BSU_00660

geneLength

1014

1017

outlinks

bsu

BSU00660

BSU_00660

The protein

Catalyzed reaction/ biological activity

phosphorylation of [[protein|RecA]] on Ser-2 [Pubmed|23634894]

phosphorylation of [[gene|tufA|EF-tu]]]] on Thr-63 during [SW|sporulation] [Pubmed|26056311]

phosphorylation of [[protein|RecA]] on Ser-2 [Pubmed|23634894]

phosphorylation of [[gene|tufA|EF-Tu]] on Thr-63 during [SW|sporulation] [Pubmed|26056311]

phosphorylation of [[protein|YabA]] on Thr-71 [pubmed|29619013]

ATP + L-seryl-[protein] --> ADP + H+ + O-phospho-L-seryl-[protein] (according to UniProt)

ATP + L-threonyl-[protein] --> ADP + H+ + O-phospho-L-threonyl-[protein] (according to UniProt)

The protein

Protein family

[SW|protein kinase] domain (according to Swiss-Prot)

[SW|protein kinase superfamily] (according to UniProt)

[SW|Ser/Thr protein kinase family] (according to UniProt)

Biological materials

Mutant

MGNA-B921 (yabT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1920 NBRP B. subtilis, Japan]

GP577 (erm), available in [SW|Jörg Stülke]'s lab

MGNA-B921 (yabT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1920 NBRP B. subtilis, Japan]

GP577 (erm), available in [SW|Jörg Stülke]'s lab

BKE00660 (Δ[[gene|yabT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTGATTGTCGTACCCGGCT, downstream forward: _UP4_TGAATGGTGCAAACTGCAGA

BKK00660 (Δ[[gene|yabT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTGATTGTCGTACCCGGCT, downstream forward: _UP4_TGAATGGTGCAAACTGCAGA

Biological materials

Expression vector

purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP391, available in [SW|Jörg Stülke]'s lab

purification from ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP823, available in [SW|Jörg Stülke]'s lab, [pubmed|20389117]

purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1408, available in [SW|Jörg Stülke]'s lab

Biological materials

lacZ fusion

translational lacZ fusion (in [[protein|pAC7]]): pGP831, available in [SW|Jörg Stülke]'s lab

translational lacZ fusion (in [SW|pAC7]): pGP831, available in [SW|Jörg Stülke]'s lab

References

Original publications

16497325, 23634894, 24731262, 25278935, 26056311

16497325, 23634894, 24731262, 25278935, 26056311, 29619013, 30671027

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|Protein kinase domain] (aa 28-286) (according to UniProt)

The protein

Structure

[PDB|6G4J] [pubmed|30671027]

Biological materials

Expression vectors

purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP391, available in [SW|Jörg Stülke]'s lab

purification from ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP823, available in [SW|Jörg Stülke]'s lab, [pubmed|20389117]

purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1408, available in [SW|Jörg Stülke]'s lab