2017-8-11 16:0:32025-05-29 01:31:57
_ec
description
Ser/Thr kinase, controls translation and DNA integrity during spore development
Ser/Thr kinase, controls [[category|Translation]] and DNA integrity during spore development
locus
BSU00660
BSU_00660
geneLength
1014
1017
outlinks
bsu
BSU00660
BSU_00660
The protein
Catalyzed reaction/ biological activity
phosphorylation of [[protein|RecA]] on Ser-2 [Pubmed|23634894]
phosphorylation of [[gene|tufA|EF-tu]]]] on Thr-63 during [SW|sporulation] [Pubmed|26056311]
phosphorylation of [[protein|RecA]] on Ser-2 [Pubmed|23634894]
phosphorylation of [[gene|tufA|EF-Tu]] on Thr-63 during [SW|sporulation] [Pubmed|26056311]
phosphorylation of [[protein|YabA]] on Thr-71 [pubmed|29619013]
ATP + L-seryl-[protein] --> ADP + H+ + O-phospho-L-seryl-[protein] (according to UniProt)
ATP + L-threonyl-[protein] --> ADP + H+ + O-phospho-L-threonyl-[protein] (according to UniProt)
The protein
Protein family
[SW|protein kinase] domain (according to Swiss-Prot)
[SW|protein kinase superfamily] (according to UniProt)
[SW|Ser/Thr protein kinase family] (according to UniProt)
Biological materials
Mutant
MGNA-B921 (yabT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1920 NBRP B. subtilis, Japan]
GP577 (erm), available in [SW|Jörg Stülke]'s lab
MGNA-B921 (yabT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1920 NBRP B. subtilis, Japan]
GP577 (erm), available in [SW|Jörg Stülke]'s lab
BKE00660 (Δ[[gene|yabT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTGATTGTCGTACCCGGCT, downstream forward: _UP4_TGAATGGTGCAAACTGCAGA
BKK00660 (Δ[[gene|yabT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTGATTGTCGTACCCGGCT, downstream forward: _UP4_TGAATGGTGCAAACTGCAGA
Biological materials
Expression vector
purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP391, available in [SW|Jörg Stülke]'s lab
purification from ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP823, available in [SW|Jörg Stülke]'s lab, [pubmed|20389117]
purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1408, available in [SW|Jörg Stülke]'s lab
Biological materials
lacZ fusion
translational lacZ fusion (in [[protein|pAC7]]): pGP831, available in [SW|Jörg Stülke]'s lab
translational lacZ fusion (in [SW|pAC7]): pGP831, available in [SW|Jörg Stülke]'s lab
References
Original publications
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|Protein kinase domain] (aa 28-286) (according to UniProt)
The protein
Structure
[PDB|6G4J] [pubmed|30671027]
Biological materials
Expression vectors
purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP391, available in [SW|Jörg Stülke]'s lab
purification from ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP823, available in [SW|Jörg Stülke]'s lab, [pubmed|20389117]
purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1408, available in [SW|Jörg Stülke]'s lab